Ribonucleoprotein particles in cellular processes.
نویسندگان
چکیده
منابع مشابه
Ribonucleoprotein particles in cellular processes
THE general way of thinking about the pathway of expression of genetic information in eukaryotes, from DNA to protein via RNA, instills a protein-centric point of view, as if the protein is the real goal and the RNA is just the intermediate. In fact, most of the RNA in the cell is not mRNA but rather part of modular structures, RNA-protein machines, that are termed ribonucleoprotein (RNP) parti...
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The LINE-1 retrotransposon (L1) encodes two proteins, ORF1p and ORF2p, which bind to the L1 RNA in cis, forming a ribonucleoprotein (RNP) complex that is critical for retrotransposition. Interactions with both permissive and repressive host factors pervade every step of the L1 life cycle. Until recently, limitations in detection and production precluded in-depth characterization of L1 RNPs. Ind...
متن کاملNuclear ribonucleoprotein particles probed in living cells.
Contacts between heterogeneous nuclear RNA (hnRNA) and protein in nuclear ribonucleoprotein particles have been photochemically crosslinked in intact HeLa or Friend erythroleukemia cell by irradiation with 254-nm light at doses of 10(1) to 10(5) ergs/mm2 (1 to 10(4) microJ/mm2). The resulting crosslinked particles were isolated and compared with conventional hnRNA . protein (hnRNP) preparations...
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Studies of the sedimentation properties of RNP(1) material from the nucleus of the amphibian oocyte have indicated (1) that there are few, if any, 78S ribosomes in the nucleus, (2) that there are smaller particles sedimenting at 50-55S and 30S, and (3) that the larger of these is the precursor of the 60S subunit of the cytoplasmic ribosomes. Although the nature of the 30S material is not comple...
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As in higher eukaryotes, heterogeneous nuclear RNA (HNRNA) in the cellular slime mold Dictyostelium discoideum is associated with proteins in the form of ribonucleoprotein particles. Mixing experiments with deproteinized hnRNA establish that the nuclear ribonucleoprotein particles are not formed artificially during isolation. In contrast to comparable material from mammalian cells (polydisperse...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1988
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.106.5.1419